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KMID : 0378019750180060747
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New Medical Journal
1975 Volume.18 No. 6 p.747 ~ p.754
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Studies on the colony inhibition assay between the histoincompatible animals
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Abstract
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Sarcomas in C3H/HeN, C57BL/6 and DDO mouse strains were induced by subcutaneous injections of 20-methylcholanthrene and cultivated in vitro. Results on tumor transplantation and colony formation on Petri dishes are summarized as follows;
1. l mg of subcutaneous injection of methylcholanthrene induced tumors in C3H/HeN, C57BL/6 and DDO mice after 8 weeks. The frequency of tumor induction in C3H/HeN was the highest. Histologically, the tumors appeared as sarcomas with numerous mitotic figures.
2. Trypsinized cell suspension of MCA-induced sarcoma of C3H/HeN were transplanted to 5 mice of C,H/HeN and palpable tumors appeared in 20 days among all of the mice. Histological finding were identical with those of the original tumor.
3. Trypsinized monolayer cell suspensions of C3H/HeN MCA-induced sarcoma culture were transplanted to 4 mice of C3H/HeN and palpable tumors arose among all of the mice within 30 days. Histological findings were identical to that of the original tumor.
4. Complete Waymouth´s Medium has been satisfactory for the establishment of MCA sarcoma cell line in vitro and maintenance of the subculture at 37°C, 5% CO2 incubator.
5. Cloning efficiencies in the cases of 104 cells/dish, 103 cells/dish and 102 cells/dish were 2.7~2.8%, 7~9% and 8~10%, respectively. The diameter of colonies was 3~6 mm as a whole.
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